HPLC Calculations

Column Efficiency - a measure of the theoretical plates (N). Where tr is the retention time of the analyte, w is peak width at a given height.        When w = half peak height, a =5.54, when w = tangent a =16.

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Capacity Factor - a measure of retention of a given compound relative to an unretained peak. Where tr is the retention time of the peak, and to is the unretained peak

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Selectivity - a measure of the resolution between two compounds.

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Resolution - a measure of the distance between two adjecent peak apex's, divided by the average base width of both peaks.

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Asymmetry - a measure of peak symmetry. Where b = apex to tail of peak, and a = apex to front of peak.  

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Tailing Factor - a measure of the symmetry of the peak. Where W_0.05 = peak width at 5% height, and f = distance form front of peak  to apex point at baseline.

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